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Bioarray Inc crna transcript labelling kit
Crna Transcript Labelling Kit, supplied by Bioarray Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crna+transcript+labeling+kit/crna+transcript+labelling+kit/pmc02640396-198-6-6
Average 90 stars, based on 1 article reviews
crna transcript labelling kit - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

In Vitro:

Article Title: Interaction Between Sleep and the Immune Response in Drosophila: A Role for the NF?B Relish
Article Snippet: .. In vitro transcription for cRNA synthesis was performed using the Enzo BioArray High Yield RNA transcript labeling Kit, and the products were subsequently cleaned up using RNeasy columns (Qiagen). .. Each sample was fragmented, and then hybridized to Affymetrix Drosophila microarrays.

Article Title: Effects of chronic morphine and morphine withdrawal on gene expression in rat peripheral blood mononuclear cells.
Article Snippet: .. Biotinylated cRNA was prepared by in vitro transcription using Enzo Bioarray High Yield RNA Transcript Labeling Kit and then fragmented. .. The fragmented and biotin-labeled cRNA samples were hybridized on Affymetrix Rat Genome RAE230_2.0 Genechips (containing 31,000 probe sets) according to the manufacturer’s instructions.

Labeling:

Article Title: Interaction Between Sleep and the Immune Response in Drosophila: A Role for the NF?B Relish
Article Snippet: .. In vitro transcription for cRNA synthesis was performed using the Enzo BioArray High Yield RNA transcript labeling Kit, and the products were subsequently cleaned up using RNeasy columns (Qiagen). .. Each sample was fragmented, and then hybridized to Affymetrix Drosophila microarrays.

Article Title: Arginase Activity Differs with Allergen in the Effector Phase of Ovalbumin- versus Trimellitic Anhydride-Induced Asthma
Article Snippet: .. The synthesis of biotin-labeled cRNA was as described in the Affymetrix manual, using the Bioarray High Yield RNA Transcript labeling ENZO Kit. .. Products of the in vitro transcription (from both before and after purification with the Qiagen RNeasy columns) were analyzed by gel electrophoresis.

Article Title: Análisis de micromatrices de ADN revela genes asociados a metástasis en líneas celulares de cáncer de próstata de rata
Article Snippet: Double-stranded cDNA was purified with phase lock gel electrophoresis (Eppendorf Scientific, Westbury, NY) and phenol-chloroform extraction, followed by ethanol precipitation. .. Upon completion of cDNA synthesis, 1μg of product was converted to biotinylated cRNA using the Enzo BioArray HighYield RNA Transcript Labeling Kit. .. Labeled cRNA was frag- mented and hybridized to the Affymetrix oligonucleotide GeneChip rat U34A arrays.

Article Title: Effects of chronic morphine and morphine withdrawal on gene expression in rat peripheral blood mononuclear cells.
Article Snippet: .. Biotinylated cRNA was prepared by in vitro transcription using Enzo Bioarray High Yield RNA Transcript Labeling Kit and then fragmented. .. The fragmented and biotin-labeled cRNA samples were hybridized on Affymetrix Rat Genome RAE230_2.0 Genechips (containing 31,000 probe sets) according to the manufacturer’s instructions.

Article Title: Functional and genomic profiling of effector CD8 T cell subsets with distinct memory fates
Article Snippet: The MEGAscript T7 kit (Ambion) was used to amplify cRNA from the cDNA. .. The cRNA was reverse transcribed with biotinylated nucleotides using the Enzo BioArray High Yield RNA transcript labeling kit in the second round of cRNA synthesis, fragmented, and hybridized on mouse 430.2 microarray chips (Affymetrix) at the Vanderbilt University Microarray Shared Resource, according to the manufacturer's protocols. .. Raw data were normalized using the RMA package built within Genespring 7.0 (Agilent Technologies), and fold-change comparisons between the groups indicated in the figures were subsequently made.

other:

Article Title: Estrogen regulated gene expression in response to neoadjuvant endocrine therapy of breast cancers: tamoxifen agonist effects dominate in the presence of an aromatase inhibitor.
Article Snippet: Estrogens (E) and estrogen receptors (ER) are implicated in breast cancer growth and are targets of hormonal therapies.. Such therapies commonly use aromatase inhibitors (AI) to block E production, or antiestrogens like tamoxifen (TAM), which targets ER.. Here we compare genes in pre-and post-treatment tumor pairs of patients with ER+ tumors, that were treated preoperatively with the AI exemestane alone, or with exemestane plus TAM.

Article Title: A PTB-dependent Program of mRNA Stability Initiates with CpG-mediated Activation of Primary B cells
Article Snippet: For RNA immunoprecipitation, protein A beads (Santa Cruz Biotechnology, Santa Cruz, CA) were coated with anti-PTB mAb (ATCC) for the microarray and either anti-PTB mAb or IgG2 b (Southern Biotech) for the RT-PCR used for binding verification.

Article Title: Molecules with effects on cellular development and function
Article Snippet: Biotin-labeled cRNA was synthesized using the BioArrayTM HighYieldTM RNA Transcript Labeling Kit, purified over RNeasy columns (Qiagen), eluted and then fragmented.

cDNA Synthesis:

Article Title: Análisis de micromatrices de ADN revela genes asociados a metástasis en líneas celulares de cáncer de próstata de rata
Article Snippet: Double-stranded cDNA was purified with phase lock gel electrophoresis (Eppendorf Scientific, Westbury, NY) and phenol-chloroform extraction, followed by ethanol precipitation. .. Upon completion of cDNA synthesis, 1μg of product was converted to biotinylated cRNA using the Enzo BioArray HighYield RNA Transcript Labeling Kit. .. Labeled cRNA was frag- mented and hybridized to the Affymetrix oligonucleotide GeneChip rat U34A arrays.

Reverse Transcription:

Article Title: Functional and genomic profiling of effector CD8 T cell subsets with distinct memory fates
Article Snippet: The MEGAscript T7 kit (Ambion) was used to amplify cRNA from the cDNA. .. The cRNA was reverse transcribed with biotinylated nucleotides using the Enzo BioArray High Yield RNA transcript labeling kit in the second round of cRNA synthesis, fragmented, and hybridized on mouse 430.2 microarray chips (Affymetrix) at the Vanderbilt University Microarray Shared Resource, according to the manufacturer's protocols. .. Raw data were normalized using the RMA package built within Genespring 7.0 (Agilent Technologies), and fold-change comparisons between the groups indicated in the figures were subsequently made.

Microarray:

Article Title: Functional and genomic profiling of effector CD8 T cell subsets with distinct memory fates
Article Snippet: The MEGAscript T7 kit (Ambion) was used to amplify cRNA from the cDNA. .. The cRNA was reverse transcribed with biotinylated nucleotides using the Enzo BioArray High Yield RNA transcript labeling kit in the second round of cRNA synthesis, fragmented, and hybridized on mouse 430.2 microarray chips (Affymetrix) at the Vanderbilt University Microarray Shared Resource, according to the manufacturer's protocols. .. Raw data were normalized using the RMA package built within Genespring 7.0 (Agilent Technologies), and fold-change comparisons between the groups indicated in the figures were subsequently made.



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